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Title: Identification of differentially expressed genes associated with semigamy in Pima cotton      
dateReleased:
02-12-2011
description:
The comparative analysis between 57-4 and Pima S-1 identified 284 genes in anthers and 1,864 genes in ovules as being differentially expressed in the semigametic genotype. Based on gene functions, 127 differentially expressed genes were common to both semigametic anthers and ovules, with 115 being consistently differentially expressed in both tissues. Nine of those genes were selected for qRT-PCR analysis, seven of which were confirmed. Furthermore, several well characterized metabolic pathways including glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms, sesquiterpenoid biosynthesis, and the biosynthesis of and response to plant hormones were shown to be affected by differentially expressed genes in the semigametic tissues. Anther and ovule tissues from 10 flowers of 57-4 and Pima S-1 was collected at zero days postanthesis (0 DPA) and placed in liquid nitrogen and stored at -80 °C. Total RNA was isolated using a hot borate method and cRNA was hybridized to the Affymetrix Cotton Genome GeneChips.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-27242
refinement:
raw
alternateIdentifiers:
27242
keywords:
functional genomics
availability:
available
types:
gene expression
name:
Gossypium barbadense
ID:
A-GEOD-8672
name:
[Cotton] Affymetrix Cotton Genome Array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-27242/E-GEOD-27242.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-27242/E-GEOD-27242.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27242
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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