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Title: ChIP-chip by array of human adult ovarian granulosa cell tumor derived cell line KGN using anti-FOXL2 antibodies      
dateReleased:
02-22-2011
description:
ChIP-on-Chip experiment using chromatin from the human adult ovarian granulosa cell tumor derived cell line KGN (Nishi et al, 2001), or from a KGN subclone overexpressing WT FOXL2 (KF3 subclone), and an isovolumic blend of our custom anti-FOXL2 polyclonal antibodies (Cocquet J et al, 2002). Non precipitated sheared matched deproteinized chromatin (Input) was used a control to estimate enrichment peaks (and thus FOXL2 binding sites) from IPed DNA. DNA from three independent ChIP assays (Input extractions) was pooled, and 100ng of DNA was linearly amplified using the Whole Genome Amplification kit (Sigma). The two ChIP-chip experiments serve as biological replicates. Moreover, 42 out of 48 targets promoters chosen from the ‘enrichment peak” list were subsequently confirmed as enrichment sites in anti-FOXL2 ChIPed DNA for subsequent experiments.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-399
refinement:
raw
keywords:
functional genomics
dateModified:
05-03-2014
creators:
Berenice A Benayoun
availability:
available
types:
gene expression
name:
Homo sapiens
name:
binding site identification
ID:
A-MEXP-1900
name:
HG18_Deluxe_Promoter_HX1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-399/E-MTAB-399.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-399/E-MTAB-399.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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