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Title: Transcription profiling by array of chicken brain and ileum after infection with H7N1 HPAIV or H7N1 LPAIV      
dateReleased:
03-01-2011
description:
Animal experiments Experiment : Chickens of group 1 were inoculated with 0.2 ml (2*105 EID50) of the H7N1 HPAIV strain, equally divided between the intranasal and intratracheal route. In the same way, animals of group 2 received 2*105 EID50 of the H7N1 LPAIV strain. Sampling: Six chickens from each group were sacrificed just before infection (controls) and at 4, 8, 16 and 24 h.p.i. From all sacrificed chickens, gross pathology of the organs was studied. Lung, trachea, ileum and brains were collected. For RNA isolation, organs were snap-frozen in liquid nitrogen and stored at -80C until use. For immonohistochemistry, samples were fixed immediately by immersion in a zinc salt-based fixative, containing 0.5 % zinc chloride, 0.5% zinc acetate in 0.1 M Tris base buffer containing 0.05 % calcium acetate, pH 7.4 for 48 h. After fixation, the tissues were dehydrated and embedded in paraffin wax. All HPAIV experiments were performed in Biosafety Level 3 facilities.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3109
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Jacob Post
availability:
available
types:
gene expression
name:
Gallus gallus
ID:
A-AFFY-103
name:
Affymetrix GeneChip Chicken Genome Array [Chicken]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3109/E-MEXP-3109.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3109/E-MEXP-3109.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress