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Title: Transcription profiling by array of human universal RNA spiked with eight arifical transcripts at different copy numbers      
dateReleased:
03-30-2011
description:
Examine the dose response characteristics of a set of eight artificial transcripts (IVT) when spiked into a Universal Human RNA background at different copy numbers. The experiment was designed to (i) evaluate the technical reproducibility of array data generated on the Agilent Whole Human 44K array with use of a set of external RNA controls (ii) employ two panels of the same external RNA controls, which differed in copy number between panels, in order to simulate normal and disease states. We employed 8 different pools which were comprised of 8 different external RNA standards in a total human reference RNA background. Each different standard was present at a different copy number within any single pool (see file: E-MEXP-2670.additional.zip). Each control was present at a different concentration in each of the 8 pools. Standard microarray analysis was performed on the data and the differences in abundance between external RNA controls determined in terms of fold change differences.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-2670
refinement:
raw
dateSubmitted:
04-08-2010
keywords:
functional genomics
dateModified:
05-02-2014
creators:
timothy m wilkes
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-MEXP-1816
name:
LGC_DD1_RNA
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-2670/E-MEXP-2670.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-2670/E-MEXP-2670.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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