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Title: Transcription profiling by array of Aspergillus fumigatus wild type and srBA knock out strains grown under hypoxic conditions      
dateReleased:
08-01-2011
description:
3 replicates of WT and srbA knockout strains grown on GMM plates, harvested spores and inoculated 5x10^5 spores/ml in 300 ml of LGMM. Cultures incubated for 10h at 28C and then shifted for 2h to 37C (both at 200rpm) so they are just germinating at the start of the treatment. Cultures were shifted to hypoxia (37C, 200rpm) and incubated for 1hr. Samples were collected by pouring them over a filter paper in a Buechner funnel and snap froze the cells immediately in liquid nitrogen, and stored the cells at -80C and lyophilized all tissue at the same time after all samples were collected. RNA extraction was then done simultaneously with all time points.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3172
refinement:
raw
keywords:
functional genomics
dateModified:
05-02-2014
creators:
Bridget M Barker
availability:
available
types:
gene expression
name:
Aspergillus fumigatus
ID:
A-MEXP-2034
name:
JCVI PFGRC Aspergillus fumigatus 22K v3 array primarily designed for strain Af293 with empty spots
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3172/E-MEXP-3172.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3172/E-MEXP-3172.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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