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Title: Adipocyte Differentiation of Immortalized Human Bone Marrow-derived Stromal Cells is Modulated by microRNA-155, microRNA-221 and microRNA-222      
dateReleased:
08-22-2011
description:
Human bone marrow mesenchymal stromal cells are capable of limited self-renewal and multi-lineage differentiation in vitro. Several studies have demonstrated that microRNAs, post-transcriptional modifiers of protein expression, play crucial roles in the regulation of these complex processes. To gain knowledge regarding the role of microRNAs in human adipocyte regulation, we examined the microRNA expression profile of the immortalized human bone marrow-derived stromal cell line hMSC-Tert20. We identified 12 microRNAs that were differentially expressed during adipogenesis, of which several have previously been shown to play important roles in adipocyte biology. The expression of miR-155, miR-221 and miR-222 decreased during the adipogenic program, suggesting that they act as negative regulators of differentiation. Interestingly, adenovirus-mediated expression of either miR-155 alone or miR-221 plus miR-222 significantly inhibited adipogenesis and repressed induction of the master regulators C/EBP and PPAR. Our study provides the first experimental evidence that miR-155, miR-221 and miR-222 function in human adipocyte differentiation. The telomerase immortalized human bone marrow-derived stromal cell line hMSC-Tert20 was differentiated towards adipocytes, and total RNA was harvested at various time points (0, 8, 24, 32, 48 and 72 hours, and 7, 14 and 21 days). All hybridizations, except for 0h, 8h and 32h, were performed twice. The expression of each miRNA at a given time point was calculated as a ratio relative to its level on day 0. No dye swaps.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-24898
refinement:
raw
alternateIdentifiers:
24898
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-11107
name:
mirVana 1.3K v1.4 201205
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24898/E-GEOD-24898.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24898/E-GEOD-24898.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24898
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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