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Title: Timecourse of total and polysome-associated mRNA levels post glucose deprivation      
dateReleased:
09-04-2011
description:
Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are lost from the cell coincident with their loss from polysomes. Second, only a very limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose re-addition in the absence of new transcription. This highly selective post-transcriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth. Sigma 1278b yeast were grown to OD600=1.0-1.1 in YPD in baffled flasks at 30C with vigorous shaking. Cells were harvested by centrifugation and resuspended in pre-warmed YPA medium lacking glucose. Cells were returned to 30C for shaking for 10,20,30,60, or 120 minutes.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-31392
refinement:
raw
alternateIdentifiers:
31392
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Saccharomyces cerevisiae Sigma1278b
ID:
A-GEOD-14148
name:
Doudna S. cerevisiae AROS YBOX 2K v 1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31392/E-GEOD-31392.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31392/E-GEOD-31392.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE31392
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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