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Title: Transcriptional responses to nanoparticulate matter in human skin-derived cancer cells      
dateReleased:
11-01-2011
description:
This study evaluated transcriptional effects of particles smaller than 100 nm of TiO2 and ZnO. Based on previous data in colon cancer cells (GSE14910), we evaluated HaCaT and Sk Mel-28 cells for transcriptional responses to 1 and 5 ug/cm2 ZnO, or 5 and 10 ug/cm2 TiO2. No particle controls were also included. Again, the most pronounced transcriptional response resulted from ZnO treatement with little responses to TiO2. We identified increased protein stress responses, decreased regulation of transcription, and responses to Zn ions. We did not observe the protein stress response and regulation of transcription with soluble Zn. Keywords: skin-derived cancer cells - response to ZnO nanoparticulate Two skin-derived cancer cell types (HaCaT and SK Mel-28) were treated with media containing the nanoparticulate, ZnCl2, or ZnO separated from the HaCaTs by a Transwell insert, and RNA was collected after 4 hrs. Generally, the RNA from 4 independent samples were combined for one microarray.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-25167
refinement:
raw
alternateIdentifiers:
25167
keywords:
functional genomics
dateModified:
05-02-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-AGIL-28
name:
Agilent Whole Human Genome Microarray 4x44K 014850 G4112F (85 cols x 532 rows)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-25167/E-GEOD-25167.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-25167/E-GEOD-25167.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE25167
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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