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Title: Total expression profiling of embryonic regions of EHF mouse embryos      
dateReleased:
11-04-2011
description:
Establishment of left-right (LR) asymmetry occurs shortly after gastrulation and utilizes a cascade of events. In the mouse, LR symmetry is broken at the node, involves signal relay to the lateral plate, and results in asymmetric organ morphogenesis. How information transmits from the node to the lateral plate remains unclear. Noting that embryos lacking Sox17 exhibit defects in both gut endoderm formation and LR patterning, we investigated a connection between these two processes. We noted an endoderm-specific absence of the critical gap junction component, Connexin43, in Sox17 mutants. Dye-coupling experiments revealed planar gap junction coupling across the gut endoderm in wild-type but not mutant embryos. The role for gap junction communication in LR patterning was confirmed by pharmacological inhibition. Collectively, our data demonstrate communication across gap junctions in gut endoderm as a mechanism for information relay between node and lateral plate critical for the establishment of LR asymmetry in mice. Total RNA isolated from embryonic regions of wild-type embryos at EHF stages, three samples per well, in triplicates
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-33353
refinement:
raw
alternateIdentifiers:
33353
keywords:
functional genomics
dateModified:
11-17-2011
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-14828
name:
Illumina MouseWG-6 v2.0 expression beadchip (Gene version based on mouseWG-6_V2_0_R3_11278593_A)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-33353/E-GEOD-33353.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-33353/E-GEOD-33353.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33353
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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