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Title: Comparison of Atlantic salmon individuals with different outcomes of cardiomyopathy syndrome (CMS)      
dateReleased:
03-29-2012
description:
Experimentally infected Atlantic salmon post-smolts exhibited different outcomes of disease (CMS). High responder (HR) fish were characterized with sustained and increased viral load and pathology in heart tissue. Low responder (LR) fish showed declining viral load from 6-10 weeks post infection (wpi) and absence of pathology. Global gene expression analyses were performed to compare these groups. Virus-responsive genes involved in early antiviral and innate immune responses were upregulated equally in LR and HR at the first stage (2-4 wpi), reflecting the initial increase in virus replication. Repression of heart muscle development indicated the early onset of pathology. By six weeks both responder groups had comparable viral loads, while increased pathology was observed in HR fish. This was reflected by induced expression of genes implicated in apoptosis and cell death, presumably related to lymphocyte regulation and survival. In contrast, LR fish showed earlier activation of NK cell-mediated cytotoxicity and NOD-like receptor signaling pathways. At the late stage of infection, increased pathology and viral load in HR was accompanied by a broad activation of genes involved in adaptive immunity and particularly T cell responses, probably reflecting the increased infiltration and homing of virus-specific T cells to the infected heart. This was in sharp contrast to LR fish, where recovery and reduced viral load was associated with a significantly reduced transcription of adaptive immunity genes and activation of genes involved in energy metabolism. Atlantic salmon post smolts (average weight 50g) were challenged with the putative CMS causing pathogen (PMCV). Heart was sampled from challenged and control fish at 2, 4, 6, 8 and 10 wpi. Heart pathology was detectable earliest at 6 wpi and from this time-point, individuals were assigned to HR and LR.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-36860
refinement:
raw
alternateIdentifiers:
36860
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Salmo salar
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-36860/E-GEOD-36860.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-36860/E-GEOD-36860.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36860
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress