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Title: Transcription profiling by array of Atlantic salmon multiple tissue reponses to Piscirickettsia salmonis      
dateReleased:
05-01-2012
description:
This experiment was aimed at understanding the early transcriptional response to the salmon pathogen Piscirikettsia salmonis at 48h post infection in multiple tissues. Agilent-based microarray platform with 4 × 44 K probes per slide (Salar_2; Agilent Design ID:025520) oligo microarray was used in this experiment. A dual-labelled experimental design was employed for the microarray hybridisations. aRNA from each experimental sample (Cy3 labelled) was competitively hybridised against a common pooled-reference sample (Cy5 labelled), which comprised equal amounts of aRNA from each of the samples used in the study. This design permits valid statistical comparisons across all treatments to be made. The entire experiment comprised 24 hybridisations - 3 tissues (head kidney, liver, muscle) × 2 treatments (SRS injection / saline control injection) × 4 biological replicates.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-685
refinement:
raw
keywords:
functional genomics
dateModified:
05-02-2014
creators:
Sam Martin
availability:
available
types:
gene expression
name:
Salmo salar
name:
compound treatment, in vivo, reference, replicate, co-expression
ID:
A-MEXP-2065
name:
Atlantic salmon_Salar2_Stirling_AgilentCustom_44K (85 col x 532 rows)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-685/E-MTAB-685.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-685/E-MTAB-685.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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