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Title: Expression data from S. cerevisiae, S288C with graded GCN5 F221A      
dateReleased:
05-02-2012
description:
S288C was transformed with plasmids expressing the GCN5 F221A mutant at varying levels. We sought to examine the global impact on gene expression using the Affymetrix yeast 2.0 arrays. TEF promoter strength varied from 32, 68 and 95% of the wild-type expression. Controls included wild-type with empty & GCN5 null strain with empty plasmid. Strains were grown for approximately 7 dblings in triplicate, followed by a mid-log phase RNA extraction. Whole cell RNA was processed for Affymetrix yeast 2.0 arrays.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-26923
refinement:
raw
alternateIdentifiers:
26923
keywords:
functional genomics
dateModified:
05-14-2012
availability:
available
types:
gene expression
name:
Saccharomyces cerevisiae
ID:
A-AFFY-47
name:
Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-26923/E-GEOD-26923.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-26923/E-GEOD-26923.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26923
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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