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Title: High-throughput RT-PCR analysis of Colon Adenocarcinoma Stem Cell Lines      
dateReleased:
07-01-2012
description:
Human early stage colon adenocarcinoma stem cell line was FACS-sorted for CCR9- and CCR9+ cell groups and differential expression was identified via Wafergen SmartChip RT-PCR gene expression profiling. CCR9- and CCR+ cells were FACS-sorted. 24 hours afterwards, cells were treated with 100ng/ml human CCL25 for 30 min. RNA was extracted from both populations using PureLink RNA Mini kit (Invitrogen) and analyzed using the SmartChip Real-Time PCR System
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-32290
refinement:
raw
alternateIdentifiers:
32290
keywords:
functional genomics
dateModified:
06-10-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-14610
name:
Wafergen Biosystems SmartChip Human Oncology Panel v2
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-32290/E-GEOD-32290.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-32290/E-GEOD-32290.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32290
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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