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Title: Transcription profiling by array of liver from proestrus and metestrus mice to investigate ER control of liver metabolic function      
dateReleased:
07-04-2012
description:
In order to elucidate the mechanism underlying ER control of liver metabolic function, we investigated the genetic programs controlled by the hepatic receptor during the physiological fluctuation of circulating E2 in adult female mice. To this aim, we used Affymetrix GeneChip Arrays and compared the expression of hepatic genes in mice in two phases of the estrous cycle: Proestrus (P) and Metestrus (M), characterized by high and low circulating E2 respectively. Analysis of data demonstrated that the expression of genes involved in lipid and cholesterol metabolism changes during the reproductive cycle. To further elucidate ER transcriptional activity at M and P, we performed a whole genome ChIP experiment followed by tiling array. We observed that at M, but not at P, recruited ERs are mostly in proximity (20 kbp) of genes involved in energy metabolism, thus strengthening the hypothesis of ER playing a bridging role between metabolism and reproduction.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3504
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Alessandro Villa
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-45
name:
Affymetrix GeneChip Mouse Genome 430 2.0 [Mouse430_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3504/E-MEXP-3504.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3504/E-MEXP-3504.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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