Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Transcription profiling by array of Enterococcus faecium to compare wild-type HM1070 strain against AsrR mutants and complemented AsrR mutants      
dateReleased:
07-09-2012
description:
Enterococcus faecium is an important opportunistic pathogen emerging in hospitals worldwide. We identified a new MarR family global regulator in E. faecium, named AsrR (antibiotic and stress response regulator). We phenotipically characterized key role for AsrR in E. faecium pathogenicity. The aim of the microarray-based experiments was to investigate the AsrR regulon in E. faecium. We constructed a mutant strain deleted for the asrR gene, we complemented the mutant and, finally, we observed genes expression in these strains in comparison with the wild-type strain. (The parental HM1070 was used to delete the asrR gene and to obtain the mutant strain. Then, the mutant strain was used to restore the asrR gene and to obtain the complemented strain.) This approach will allow us to identify the genes regulated by AsR to clarify its role in E. faecium.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3528
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Francois Lebreton
availability:
available
types:
gene expression
name:
Enterococcus faecium
ID:
A-MEXP-2172
name:
UMCU E. faecium 15K v1 (alternate layout)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3528/E-MEXP-3528.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3528/E-MEXP-3528.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.