Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: ChIP-chip by array of human T-cell acute lmphoblastic leukaemia (T-ALL) cells using anti-H3K27me3 antibody      
dateReleased:
07-17-2012
description:
Relative H3K27me3 enrichment, assessed by ChIP-on-chip, in two TLX positive and two TLX negative T Acute Lymphoblastique Leukemia (T-ALL) samples. Acute lymphoblastic leukemias (ALLs) are characterized by multi-step oncogenic processes leading to cell differentiation arrest and proliferation. Specific abrogation of maturation blockage constitutes a promising therapeutic option in cancer, which requires precise understanding of the underlying molecular mechanisms. We show that the cortical thymic maturation arrest in T-lineage ALL that over-express TLX1 or TLX3 is due to binding of TLX1/TLX3 to ETS1, leading to repression of the T cell receptor (TCR) alpha enhanceosome activity and blocked TCR-Jalpha rearrangement. TLX1/TLX3 abrogation or enforced TCRalpha/beta expression leads to TCRalpha rearrangement and apoptosis. Importantly, the auto-extinction of clones carrying TCRalpha-driven TLX1 expression supports TLX 'addiction' in TLX-positive leukemias and provides further rationale for targeted therapy based on disruption of TLX1/TLX3.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3527
refinement:
raw
keywords:
functional genomics
dateModified:
01-26-2012
creators:
Salvatore spicuglia
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-MEXP-2178
name:
Agilent human promoter custom array version 2 (ChIP/CH3 2x400k SurePrint G3 personnal), 398 cols x 1056 rows
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3527/E-MEXP-3527.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3527/E-MEXP-3527.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
Similar Datasets

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.