biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | ChIP-chip by array of human T-cell acute lmphoblastic leukaemia (T-ALL) cells using anti-H3K27me3 antibody
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| dateReleased: |
07-17-2012
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| description: |
Relative H3K27me3 enrichment, assessed by ChIP-on-chip, in two TLX positive and two TLX negative T Acute Lymphoblastique Leukemia (T-ALL) samples. Acute lymphoblastic leukemias (ALLs) are characterized by multi-step oncogenic processes leading to cell differentiation arrest and proliferation. Specific abrogation of maturation blockage constitutes a promising therapeutic option in cancer, which requires precise understanding of the underlying molecular mechanisms. We show that the cortical thymic maturation arrest in T-lineage ALL that over-express TLX1 or TLX3 is due to binding of TLX1/TLX3 to ETS1, leading to repression of the T cell receptor (TCR) alpha enhanceosome activity and blocked TCR-Jalpha rearrangement. TLX1/TLX3 abrogation or enforced TCRalpha/beta expression leads to TCRalpha rearrangement and apoptosis. Importantly, the auto-extinction of clones carrying TCRalpha-driven TLX1 expression supports TLX 'addiction' in TLX-positive leukemias and provides further rationale for targeted therapy based on disruption of TLX1/TLX3.
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| privacy: |
not applicable
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| aggregation: |
instance of dataset
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| ID: |
E-MEXP-3527
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| refinement: |
raw
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| keywords: |
functional genomics
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| dateModified: |
01-26-2012
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| creators: |
Salvatore spicuglia
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| availability: |
available
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| types: |
gene expression
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| name: |
Homo sapiens
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| ID: |
A-MEXP-2178
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| name: |
Agilent human promoter custom array version 2 (ChIP/CH3 2x400k SurePrint G3 personnal), 398 cols x 1056 rows
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3527/E-MEXP-3527.raw.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3527/E-MEXP-3527.processed.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| abbreviation: |
EBI
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| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
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| name: |
European Bioinformatics Institute
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| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
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| name: |
ArrayExpress
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