dateReleased: |
07-26-2012
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description: |
Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa. Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..
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privacy: |
not applicable
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aggregation: |
instance of dataset
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ID: |
E-GEOD-39687
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refinement: |
raw
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alternateIdentifiers: |
39687
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keywords: |
functional genomics
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dateModified: |
09-30-2015
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availability: |
available
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types: |
gene expression
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name: |
Oryza sativa
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ID: |
A-AFFY-126
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name: |
Affymetrix GeneChip Rice Genome Array [Rice]
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-39687/E-GEOD-39687.raw.1.zip |
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ArrayExpress
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gzip compressed
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none
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-39687/E-GEOD-39687.processed.1.zip |
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gzip compressed
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accessType: |
download
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authentication: |
none
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none
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accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39687 |
storedIn: |
Gene Expression Omnibus
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not compressed
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format: |
HTML
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accessType: |
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true
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none
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none
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abbreviation: |
EBI
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homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
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name: |
European Bioinformatics Institute
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homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
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name: |
ArrayExpress
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