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Title: Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line (Human cancer array)      
dateReleased:
08-01-2012
description:
75% ovarian epithelial tumor overexpresses Lewis y antigen, which notably strenthen such malignant biological behaviors as metastasis, drug resistance and proliferation. The key enzyme of Lewis y synthesis is human fucosyltransferase gene 1(hfut1). Through transfecting hfut1 into Lewis y low expressing cell line RMG-I, we got a pair of cell lines. We hypothesized that Lewis y antigen plays a major role in ovarian canceration . Using gene arrays, we comprehensively profiled mRNA differential expression between fut1-pretransfected and posttransfected clear cell of human epithelial OvCa. We expected that over-expression of lewis y antigen would lead to higher expression of genes associated with growth, metastasis,proliferation and so on. we established ovarian tumor cell line RMG-I-H by stably transfecting human fucosyltransferase gene 1 into cell line RMG-I. RMG-I-H overexpresses Lewis y antigen, which is known as a cancer-related tetrasaccharide antigen. We profiled for tumor-associated gene expression both cells pretransfected and posttransfected.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-39216
refinement:
raw
alternateIdentifiers:
39216
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-15784
name:
Human Cancer Array 11K
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-39216/E-GEOD-39216.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-39216/E-GEOD-39216.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39216
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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