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Title: The PerA regulon in Enterococcus faecalis      
dateReleased:
08-16-2012
description:
Transcriptional profiling of E. faecalis E99 WT and an isogenic ΔperA strain grown in THB + 1% glucose. Pathogenic E. faecalis are enriched for a pathogenicity island (PAI). This 150-kb island harbors a number of well characterized virulence genes plus a number of determinants of unknown function including one encoding a transcriptional regulator, designated PerA. In this work, we show that PerA coordinately regulates both metabolic and virulence genes, and influences the platelet binding ability of E. faecalis. Finally, we show that PerA responds to bicarbonate, an intestinal ion frequently used by pathogens to determine the site of infection. Together, these results indicate that PerA is a global transcriptional regulator that coordinately regulates genes responsible for enterococcal pathogenicity. These findings highlight a novel feature of PAI-mediated virulence regulation, namely the coordinate regulation of metabolic and virulence factors in the core genome by a horizontally acquired PAI-encoded transcriptional regulator. RNA was obtained from E. faecalis E99 ΔperA from mid-log (OD600 0.05), late log (OD600 0.5) and stationary phase (OD600 1.0) and compared to E. faecalis E99 WT RNA extracted from the same growth phases. Cultures were grown in THB + 1% glucose.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-31538
refinement:
raw
alternateIdentifiers:
31538
keywords:
functional genomics
dateModified:
09-26-2012
availability:
available
types:
gene expression
name:
Enterococcus faecalis
ID:
A-GEOD-6702
name:
Affymetrix Custom Array - enterocha520147F
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31538/E-GEOD-31538.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31538/E-GEOD-31538.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE31538
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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