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Title: Gene expression profiling in murine mesenchymal stem cells treated with local anesthetics (ropivacaine)      
dateReleased:
08-31-2012
description:
Mesenchymal stem cells (MSCs) are recruited from the blood stream to tumors, healing wounds or sites of tissue injury by multiple growth factors and chemokines where they differentiate into e.g. fibroblasts or endothelial cells. Engrafted at the sites of tissue damage, they secrete growth factors and cytokines that facilitate healing. Inhibition of MSC proliferation by prolonged application of local anesthetics (commonly used after surgery) could delay wound closure and promote wound dehiscence. Culture-expanded bone-marrow-derived murine MSCs wewre treated in vitro with 100 uM ropivacaine for 24 hours (4 samples). Untreated MSCs served as controls.The collected cells were immediately frozen in liquid nitrogen and later used for RNA isolation and subsequent microarray hybridization. Gene-level analysis was performed. Exon-level analysis will be also performed.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-31827
refinement:
raw
alternateIdentifiers:
31827
keywords:
functional genomics
dateModified:
09-26-2012
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-14363
name:
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [CDF: Brainarray Version 11.0.1, MoEx10stv1_Mm_ENSG]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31827/E-GEOD-31827.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-31827/E-GEOD-31827.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE31827
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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