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Title: Transcriptional profiling of bulge stem cells      
dateReleased:
10-19-2012
description:
In adult skin, each hair follicle contains a reservoir of stem cells (the bulge), which can be mobilized to regenerate the new follicle with each hair cycle and to reepithelialize epidermis during wound repair. Here we report new methods that permit their clonal analyses and engraftment and demonstrate the two defining features of stem cells, namely self-renewal and multi-potency. We also show that, within the bulge, there are two distinct populations, one of which maintains basal lamina contact and temporally precedes the other, which is suprabasal and arises only after the start of the first postnatal hair cycle. This spatial distinction endows them with discrete transcriptional programs, but surprisingly, both populations are growth inhibited in the niche but can self-renew in vitro and make epidermis and hair when grafted. These findings suggest that the niche microenvironment imposes intrinsic “stemness” features without restricting the establishment of epithelial polarity and changes in gene expression. FACS was used to isolate pure populations of living total K14-GFP-actin expressing cells and K14-GFP-actin α6high CD34high cells from postnatal d49 mouse back skins for RNA extraction and hybridization on Affymetrix microarrays. By comparing the transcriptional profile of FACS-isolated GFP α6high CD34high expressing cells with FACS-isolated total GFP cells, we determined the genes differentially regulated in basal bulge stem cells in telogen stage comparing to total epidermis
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-41704
refinement:
raw
alternateIdentifiers:
41704
keywords:
functional genomics
dateModified:
10-29-2012
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-23
name:
Affymetrix GeneChip Mouse Expression Array MOE430A [MOE430A]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-41704/E-GEOD-41704.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-41704/E-GEOD-41704.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41704
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress