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Title: Revisiting Global Gene Expression Analysis (Microarray)      
dateReleased:
10-25-2012
description:
Gene expression analysis is a widely used and powerful method for investigating the transcriptional behavior of biological systems, for classifying cell states in disease and for many other purposes. Recent studies indicate that common assumptions currently embedded in experimental and analytical practices can lead to misinterpretation of global gene expression data. We discuss these assumptions and describe solutions that should minimize erroneous interpretation of gene expression data from multiple analysis platforms. RNA was extracted from cells expressing low or high levels of c-Myc. A panel of synthetic RNA's was added to these populations, based on cell number. Total RNA was then extracted and processed on Affymetrix microarrays in biological duplicate.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-40782
refinement:
raw
alternateIdentifiers:
40782
keywords:
functional genomics
dateModified:
11-09-2012
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-16043
name:
GeneChip® PrimeView™ Human Gene Expression Array (with External spike-in RNAs)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-40782/E-GEOD-40782.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-40782/E-GEOD-40782.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE40782
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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