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Title: Transcription profiling by array of maternal RNA in Atlantic cod (Gadus morhua) eggs and embryos after female broodstock stress      
dateReleased:
11-01-2012
description:
A stable supply of viable eggs and embryos is crucial for successful farming of Atlantic cod. Broodstock stress can have negative effects, but little is known about the molecular mechanisms that cause abnormal development. Maternally transferred mRNAs have been shown to be essential for normal development, and stress may therefore influence their expression and the subsequent embryonic development. We investigated if mimicked stress in prespawning Atlantic cod females affects mRNA concentrations in eggs and embryos, and if this can be linked to egg/embryo viability, and if we could identify potential molecular markers for stress and egg/embryo viability. Consequently, 3 weeks prior to expected peak spawning, 20 females were intraperitoneally implanted with either cortisol-containing or cortisol-free (sham) osmotic pumps. At peak spawning all individuals were stripped and eggs were fertilized and incubated until hatching. Samples were collected from unfertilized eggs and embryos for gene expression profiling.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-1296
refinement:
raw
dateSubmitted:
07-13-2012
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Gadus morhua
name:
compound treatment
ID:
A-MEXP-2226
name:
Atlantic Cod Agilent Custom array 4x44K
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1296/E-MTAB-1296.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1296/E-MTAB-1296.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress