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Title: S. coelicolor LY2517 R5- medium time course study (Culture # 1)      
dateReleased:
12-19-2012
description:
Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO2517::kan mutant strain (LY2517) and compare it to the wild-type M145 strain. SCO2517 is a putative two-component system response regulator. The time-course study involved analysis of 6 samples (15h, 22h, 24h, 26h, 35h, 43h). All samples were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-21809
refinement:
raw
alternateIdentifiers:
21809
keywords:
functional genomics
dateModified:
05-02-2014
availability:
available
types:
gene expression
name:
Streptomyces coelicolor
ID:
A-GEOD-4908
name:
Scoelicolor_UMN_Def_v1.0
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-21809/E-GEOD-21809.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-21809/E-GEOD-21809.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE21809
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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