dateReleased: |
02-22-2013
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description: |
modENCODE_submission_3888 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to and EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Su(var) 3-9 06 17 mutant(official name : Su(var)3-9^06/Su(var)3-9^17 genotype : Su(var)3-9^06/Su(var)3-9^17 outcross : n/a transgene : n/a tags : X-ray tag : GFP description : Stocks with the Su(var)3-9^06 and Su(var)3-9^17 alleles were maintained as heterozygous stocks over a third chromosome balancer. To generate the transheterozygotes description : individuals from the heterozygous stocks were crossed together. The F1 individuals lacking the balancer chromosome were used for these experiments. made_by : Stocks were obtained from Gary Karpen ); Developmental Stage: 3rd Instar Larvae; Genotype: Su(var)3-9^06/Su(var)3-9^17; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Su(var) 3-9 06 17 mutant(official name : Su(var)3-9^06/Su(var)3-9^17 genotype : Su(var)3-9^06/Su(var)3-9^17 outcross : n/a transgene : n/a tags : X-ray tag : GFP description : Stocks with the Su(var)3-9^06 and Su(var)3-9^17 alleles were maintained as heterozygous stocks over a third chromosome balancer. To generate the transheterozygotes description : individuals from the heterozygous stocks were crossed together. The F1 individuals lacking the balancer chromosome were used for these experiments. made_by : Stocks were obtained from Gary Karpen ); Antibody H3K36me3 abcam (target is H3K36me3); Developmental Stage 3rd Instar Larvae
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privacy: |
not applicable
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aggregation: |
instance of dataset
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ID: |
E-GEOD-44501
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refinement: |
raw
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alternateIdentifiers: |
44501
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keywords: |
functional genomics
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dateModified: |
05-03-2014
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availability: |
available
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types: |
gene expression
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name: |
Drosophila melanogaster
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ID: |
A-AFFY-146
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name: |
Affymetrix GeneChip Drosophila Tiling 2.0R Array [Dm_tiling2_MR]
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-44501/E-GEOD-44501.raw.1.zip |
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ArrayExpress
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qualifier: |
gzip compressed
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format: |
TXT
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accessType: |
download
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authentication: |
none
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authorization: |
none
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-44501/E-GEOD-44501.processed.1.zip![]() |
storedIn: |
ArrayExpress
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qualifier: |
gzip compressed
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format: |
TXT
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accessType: |
download
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authentication: |
none
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authorization: |
none
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accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE44501 |
storedIn: |
Gene Expression Omnibus
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qualifier: |
not compressed
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format: |
HTML
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accessType: |
landing page
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primary: |
true
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authentication: |
none
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authorization: |
none
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abbreviation: |
EBI
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homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
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name: |
European Bioinformatics Institute
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homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
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name: |
ArrayExpress
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H3K9me3 abcam.D.mel 3rd Instar Larvae Nuclei Su(var) 3-9 06_17 Mutant...
H3K9me1 (new lot 2).D.mel 3rd Instar Larvae Nuclei Su(var) 3-9 06_17 Mutant...
HP1 wa191.D.mel 3rd Instar Larvae Nuclei Su(var) 3-9 06_17 Mutant...
H3K9me2-Ab2 (new lot).D.mel 3rd Instar Larvae Nuclei Su(var) 3-9 06_17 Muta...
H3K9me2-Ab2 (new lot).D.mel 3rd Instar Larvae Nuclei Su(var) 3-9 Mutant...