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Title: Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein      
dateReleased:
04-06-2013
description:
A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was separated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interactions, rabbit polyclonal IgG to C. jejuni was used, while unspecific binding to the epitope sequence was checked using rabbit polyclonal IgG to Salmonella enterica.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-45556
refinement:
raw
alternateIdentifiers:
45556
keywords:
functional genomics
dateModified:
04-22-2013
creators:
Sebastian Hoppe
availability:
available
types:
gene expression
name:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
ID:
A-GEOD-16889
name:
IBMT Campylobacter jejuni alanine scan
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-45556/E-GEOD-45556.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-45556/E-GEOD-45556.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45556
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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