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Title: Transcription profiling by array of PUFA-treated rats to better understand the mechanisms of miRNAs in PUFA-modulated cellular pathways      
dateReleased:
04-22-2013
description:
To better understand the mechanisms of miRNAs in PUFA-modulated cellular pathways, we compared physical signs and immune statuses of PUFA-treated rats. The results indicated that omega-3 PUFAs decreased production of pro-inflammatory cytokines such as interleukin-6, C-reactive protein, and tumor necrosis factor-a, at the same time, increased proportion of CD8+ suppressor T cells and regulatory T cells in rats. The changes of these biological parameters showed that the PUFA diet-induced autoimmune (AI)-prone and AI-averse rat models were successfully established. As following steps, differentially expressed miRNAs were filtered in peripheral serum by microarray assay and validated in PBMC, liver, visceral fat, and pituitary gland by stem-loop real-time quantitative-PCR (RT-qPCR). Biological databases and computational algorithms were used to classify the potential target genes of these PUFA-induced differentially expressed miRNAs.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-1615
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Zheng Zheng
availability:
available
types:
gene expression
name:
Rattus norvegicus
ID:
A-MEXP-2004
name:
Exiqon miRCURY LNA microRNA Array 6th generation - hsa mmu and rno - miRBase 16.0 annotation
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1615/E-MTAB-1615.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1615/E-MTAB-1615.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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