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Title: Differential expression analysis of T. thermophilus HB8 wild-type strain in response to addition of silicic acid and removal of iron      
dateReleased:
05-14-2013
description:
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 60 min after the addition of silicic acid and removal of iron. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TM medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl and 1% Castenholz' basal salt solution, which was adjusted to pH 7.2 with NaOH. The cells (1 mL) were inoculated into 250 mL of the synthetic medium and then cultivated at 70°A600 value being ~0.60). Then the 600 ppm of silicic acid was added into the medium or removal of iron, and continued cultivation. Cells were collected at 60 min time point after the change of medium, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-46868
refinement:
raw
alternateIdentifiers:
46868
keywords:
functional genomics
dateModified:
05-22-2013
availability:
available
types:
gene expression
name:
Thermus thermophilus HB8
ID:
A-GEOD-4902
name:
Riken Thermus thermophilus HB8 4K mRNA array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46868/E-GEOD-46868.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46868/E-GEOD-46868.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46868
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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