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Title: New target genes of MITF-induced microRNA-211 contribute to melanoma cell invasion      
dateReleased:
08-01-2013
description:
IGR39 cells, seeded at a density of 6x104 cells/well in 12-well culture plates, were transfected with 5 nM of miR-211 mimic or negative control (NCM) as described in the main text. Samples for total RNA extraction were collected 48h after transfection. RNA quality was assessed using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent, Paolo Alto, USA). Gene expression profiling experiments using GeneChip® Human Gene 2.0 ST arrays and miRNA profiling using GeneChip® miRNA arrays (Affymetrix, Santa Clara, CA, USA) were performed on two independent samples at the CRP-Santé Microarray Unit (Luxembourg) as described before (Reinsbach et al., 2012) and according to standard protocols. Standard pipeline from the Partek® Genomics SuiteTM (Partek GS) software was used for analysis of data files. Lists of genes were generated by pair-wise comparison of expression data sets (negative control vs. mimic treated samples at 48h).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3749
refinement:
raw
keywords:
functional genomics
dateModified:
08-01-2013
creators:
Arnaud Muller
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-AFFY-141
name:
Affymetrix GeneChip Human Gene 1.0 ST Array [HuGene-1_0-st-v1]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3749/E-MEXP-3749.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3749/E-MEXP-3749.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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