Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: B lymphocyte activation induces DNA methylation reprogramming and establishes a common epigenetic signature in memory B and plasma cell compartments [gene expression profiling]      
dateReleased:
08-13-2013
description:
DNA methylation profiling of human B cell populations representing a developmental series before and after immune activation. The B cells are derived from inflamed tonsils. The B cells were already activated in vivo in patients, therefore there was no need to stimulate the B cells after isolation. Gene expression profiling was also performed from the same samples. Four B cell subsets: Naïve, germinal center B cells (GC), memory B cells, and plasma cells (PC) were purified by FACS ex vivo from tonsils of 8 human subjects. Methylated CpG island Recovery assay (MIRA) was utilized to enrich for methylated DNA fragments from each sample for microarray analysis. RNA was copurified from the same cell samples for gene expression profiling on microarray. *** This Series reports gene expression profiling data. ***
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-24863
refinement:
raw
alternateIdentifiers:
24863
keywords:
functional genomics
dateModified:
08-21-2013
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-10191
name:
NimbleGen Homo sapiens HG18 Expression Array (12x135k)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24863/E-GEOD-24863.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24863/E-GEOD-24863.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24863
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.