Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Microarray analysis of deletants of two transcription factor B in the hyperthermophilic archaeon, Thermococcus kodakarensis      
dateReleased:
08-14-2013
description:
Thermococcus kodakarensis, which can grow in a wide range of temperatures, from 60°C to 100°C, optimally at 85°C, has two transcription factor Bs, TFB1 and TFB2, both of which are basal transcription factor. TFB1 is unique to hyperthermophiles, but TFB2 is widely conserved in Euryarchaeota. To screen the genes dependent on each TFB in a temperature dependent manner, we investigated the transcriptional profilings of each TFB deletant strain grown at each temperature, 60˚C, 85˚C, and 93˚C, by comparing with the host strain, KU216. Data analyses showed that TFB1 apparently controls the expression of genes essential for motility and adhesion, such as flagellin genes, at 85˚C and 93˚C, but TFB2 regulates genes involved in basal metabolism including amino acid biosynthesis. Two-condition experiment, KU216 vs. DTF1 cells and KU216 vs. DTF2. Technical replicates: 2 DTF1 at 60˚C, 2 DTF1 at 85˚C, 2 DTF1 at 93˚C, 2 DTF2 at 60˚C, 2 DTF2 at 85˚C, 2 DTF2 at 93˚C, independently measured. One replicate per array.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-49841
refinement:
raw
alternateIdentifiers:
49841
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Thermococcus kodakarensis
ID:
A-GEOD-17565
name:
Thermococcus kodakarensis microarray
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-49841/E-GEOD-49841.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-49841/E-GEOD-49841.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE49841
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.