Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Myb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast      
dateReleased:
08-14-2013
description:
The TTAGGG motif is common to two seemingly unrelated dimensions of chromatin function – the vertebrate telomere repeat and the promoter regions of many Schizosaccharomyces pombe genes, including all of those encoding canonical histones. The essential S. pombe protein Teb1 contains two Myb-like DNA binding domains related to those found in telomere proteins and binds the human telomere repeat sequence TTAGGG. Here we analyze Teb1 binding throughout the genome and the consequences of reduced Teb1 function. ChIP on chip analysis reveals robust Teb1 binding at many promoters, notably including all of those controlling canonical histone gene expression. A hypomorphic allele, teb1-1, confers reduced binding and reduced levels of histone transcripts. teb1-1 cells also show severe defects in the G1-arrest-associated clipping of histones, most likely due to a role for Teb1 in promoting expression of the protease Isp6. Prompted by previously suggested connections between histone expression and centromere identity, we examined localization of the centromeric histone H3 variant Cnp1 and found reduced centromeric binding along with reduced centromeric silencing. These data identify Teb1 as a master regulator of histone levels and centromere identity.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-1253
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
availability:
available
types:
gene expression
name:
Schizosaccharomyces pombe
ID:
A-MEXP-1462
name:
Agilent S. Pombe Whole Genome ChIP-on-Chip Microarray 4x44K 015424 G4810A (266 Col x 170 Row)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1253/E-MTAB-1253.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1253/E-MTAB-1253.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
Similar Datasets

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.