biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis (smRNA-seq)
|
| dateReleased: |
08-16-2013
|
| description: |
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) negatively regulate their targets by 1) repressing translation, 2) endonucleolytic RNA cleavage, or 3) DNA methylation resulting in transcriptional silencing. P-body/decapping components are likely required for translational repression, but are not known to function in other posttranscriptional regulatory pathways or to affect smRNA levels. Here, we show that the P-body/decapping protein DCP5 is required for miRNA-mediated translational repression but not cleavage, and to regulate the transcription of specific miRNAs. We find that this protein also affects the abundance of tRNA-derived smRNAs. Significantly, DCP5 is required for the transcriptional silencing and DNA methylation of numerous transposable/repetitive elements and imprinted genes, indicating that it is a novel component of the RNA-directed DNA methylation pathway. Our results demonstrate that DCP5 and likely the P-body itself are required for multiple smRNA-mediated silencing pathways and provide the first evidence for the spatial separation of translational inhibition and cleavage by miRNAs. small RNA (smRNA) expression comparison between wildtype (Col-0) and dcp5 mutant plants in Arabidopsis
|
| privacy: |
not applicable
|
| aggregation: |
instance of dataset
|
| ID: |
E-GEOD-28512
|
| refinement: |
raw
|
| alternateIdentifiers: |
28512
|
| keywords: |
functional genomics
|
| dateModified: |
06-02-2014
|
| availability: |
available
|
| types: |
gene expression
|
| name: |
Arabidopsis thaliana
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-28512/E-GEOD-28512.raw.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-28512/E-GEOD-28512.processed.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28512 |
| storedIn: |
Gene Expression Omnibus
|
| qualifier: |
not compressed
|
| format: |
HTML
|
| accessType: |
landing page
|
| primary: |
true
|
| authentication: |
none
|
| authorization: |
none
|
| abbreviation: |
EBI
|
| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
|
| name: |
European Bioinformatics Institute
|
| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
|
| name: |
ArrayExpress
|