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Title: Campylobacter jejuni transcriptomic response to benzylisothiocyanate      
dateReleased:
09-19-2013
description:
Campylobacter jejuni is a widespread pathogen responsible for most of the food-borne gastrointestinal diseases in Europe. For pathogen control in the food industry, the use of natural antimicrobial molecules is a promising strategy to avoid antibiotic treatments. Isothiocyanates are natural antimicrobial compounds which also display anti-cancer activity. Several studies described the chemoprotective effect of isothiocyanates on eukaryotic cells, but the antimicrobial mechanism is still poorly understood. We investigated the early cellular response of C. jejuni to benzylisothiocyanate (BITC) by both transcriptomic and physiological (respirometry, ATP content measurements and isolations of aggregated proteins). To characterize the transcriptomic early response to benzylisothiocyanate, C. jejuni NCTC11168 were grown in 100 ml flasks containing 25 ml of MEMα medium plus 20 mM sodium pyruvate. At mid-log phase, 2µg/mL benzylisothiocyanate in ethanol, or the same volume of ethanol (control) was added to the flasks for 10 or 15 min prior to total RNA extraction and purification. Samples were then processed for microarray hybridization. Microarray data was acquired from two (10 minutes assay) or three (15 minutes assay) independent biological replicates and 6 to 9 technical replicates for each biological replicate (total number of measurement per gene = 42).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-45823
refinement:
raw
alternateIdentifiers:
45823
keywords:
functional genomics
dateModified:
05-03-2014
availability:
available
types:
gene expression
name:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
ID:
A-GEOD-6293
name:
Stintzi Lab Campylobacter jejuni 1.9k array, version 4.0
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-45823/E-GEOD-45823.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-45823/E-GEOD-45823.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45823
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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