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Title: The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Neonates      
dateReleased:
09-24-2013
description:
To study the gene expression during EV71 infection, we have employed whole Rhesus Monkey genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish infection process. Rhesus monkey peripheral blood from the experiment or control donors was isolated, extracted the RNA and screened by microarray. The results indicated that the expression levels of more than 500 functional genes associated with multiple pathways were modulated. To confirm the results of the microarray analysis, 25 individual genes were random selected and were analyzed using real-time RT-PCR amplification. The expression levels of genes associated with immune inflammatory responses were up-regulated during the period from day 4 to 10 post-infection. The expressions of two genes (TAC1 and IL17A), which play major roles in inflammatory reactions, were remarkably up-regulated during the infection period. Enterovirus 71 infection induced gene expression in rhesus monkey peripheral blood mononuclear cells was measured at 4, 7 and 10 days after viral infection. Three samples from two experiment donors and one control donor were performed at each time (4, 7 or 10 days). To exclude the individual difference, mix the two experiment samples at each time, and mix the three control samples.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-51103
refinement:
raw
alternateIdentifiers:
51103
keywords:
functional genomics
dateModified:
06-03-2014
availability:
available
types:
gene expression
name:
Macaca mulatta
ID:
A-GEOD-16027
name:
Agilent-026806 M. mulatta (Rhesus) Oligo Microarray v2 (Probe Name Version)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-51103/E-GEOD-51103.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-51103/E-GEOD-51103.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51103
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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