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Title: Transcription profiling by array of mouse neural progenitor cells isolated from forebrains of E13.5 dpc mouse embryos with wild-type Miz1 or homozygous for Miz1-delta-POZ      
dateReleased:
10-01-2013
description:
To isolate neuronal progenitor cells (NPCs), forebrains of E13.5 Miz1+/+ or Miz1-delta-POZ embryos were cut in small pieces, digested with trypsin and filtered through sterile gauze. Cells were cultivated in 2:1 DMEM/F12 supplemented with 1xB27 (Life technologies), 20 ng/ul EGF (Biomol), 20 ng/?l basic FGF (Biomol), 1 ug/ml fungizone (Gibco) and Penicillin/Streptomycin (PAA). NPCs were passaged every seven days. RNA expression of different genotypes was compared in sec. and quart. neurospheres.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3883
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Anneli Gebhardt
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-MEXP-2114
name:
Agilent SurePrint G3 Mouse GE 8x60K Microarray 028005 ( 85 cols x 532 rows )
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3883/E-MEXP-3883.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3883/E-MEXP-3883.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress