Title: | MNase-seq of budding yeast cells to determine nucleosome modelling over a Msn2-activation time-course
![]() |
dateReleased: |
10-12-2013
|
description: |
The aim of this experiment was to determine, using MNAse-Seq, how nucleosomes get remodeled during an Msn2 activation timecourse genome-wide. Diploid strain EY2807/ASH79 with genotype TPK1M164G TPK2M147G TPK3M165G msn4::TRP1/LEU2 MSN2-mCherry NHP6a-iRFP::kanMX was used. This strain is PKAas, so upon addition of the inhibitor 1-NM-PP1, Msn2 translocates to the nucleus and activates gene expression. In this experiment, the diploid strain was exposed to 3 uM 1-NM-PP1 for 0, 5, 10, 20 and 40 min and nucleosome positions determined using by crosslinking, MNAse treatment, nucleosomal DNA purification and paired-end high-throughput sequencing (Illumina). Results from transcriptional profiling of yeast with or without Msn2 expression were also deposited at ArrayExpress under accession number E-MTAB-1945 ( ).
|
privacy: |
not applicable
|
aggregation: |
instance of dataset
|
ID: |
E-MTAB-1950
|
refinement: |
raw
|
keywords: |
functional genomics
|
dateModified: |
09-09-2014
|
creators: |
Anders S Hansen
|
availability: |
available
|
types: |
gene expression
|
name: |
Saccharomyces cerevisiae
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1950/E-MTAB-1950.raw.1.zip |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1950/E-MTAB-1950.processed.1.zip |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
abbreviation: |
EBI
|
homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
|
name: |
European Bioinformatics Institute
|
homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
|
name: |
ArrayExpress
|