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Title: Transcription profiling by array of Escherichia coli to study the persister phenotype      
dateReleased:
10-13-2013
description:
When bacteria are challenged with antimicrobials, small numbers of cells may survive treatment. These so-called persister cells are not mutants, but are a set of phenotypic variants which, after re-growth exhibit the same susceptibility properties as their progenitor populations. The presence of persisters may determine the effective therapeutic dose of antibiotics and account for the intractability of biofilm-related infections. In the current investigation, Escherichia coli persister cells were isolated without prior exposure to antimicrobials, using fluorescence-assisted cell sorting of a strain, labelled with a chromosomal green fluorescent protein marker. Levels of persistence were inversely proportional to fluorescence intensity in the gfp-tagged E. coli population, and separated persister cells showed enhanced expression of two groups of genes located in the e14 and CP4-6 cryptic prophage regions when analysed by DNA microarray.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-3511
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Leo Zeef
availability:
available
types:
gene expression
name:
Escherichia coli
ID:
A-AFFY-29
name:
Affymetrix GeneChip E.coli Antisense Genome Array [Ecoli_ASv2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3511/E-MEXP-3511.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3511/E-MEXP-3511.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress