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Title: Single cell analysis of the transcriptomic heterogeneity of ground state pluripotency      
dateReleased:
10-21-2013
description:
This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes, e.g. Nanog, in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study, we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum/LIF and 2i/LIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-ERAD-186
refinement:
raw
keywords:
functional genomics
dateModified:
05-17-2016
creators:
The Wellcome Trust Sanger Institute
availability:
available
types:
gene expression
name:
Mus Musculus
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-ERAD-186/E-ERAD-186.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-ERAD-186/E-ERAD-186.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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