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Title: RNA-seq of pure fractions of nuclear and cytoplasmic nascent or mature RNA with or without polyA enrichment from different cellular compartments      
dateReleased:
10-22-2013
description:
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human brain samples and sequenced on the SOLiD system. We analyzed the data to look for differences in levels of nascent transcription and mature mRNAs between the different samples.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-1898
refinement:
raw
keywords:
functional genomics
dateModified:
06-03-2014
creators:
Adam Ameur
availability:
available
types:
gene expression
name:
Homo sapiens
name:
cell type comparison
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1898/E-MTAB-1898.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1898/E-MTAB-1898.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress