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Title: Transcription profiling by array of overexpression of the Rv0805 phosphodiesterase elicits a cAMP-independent transcriptional response      
dateReleased:
11-02-2013
description:
The Rv0805 gene in Mycobacterium tuberculosis encodes a metallophosphoesterase which shows cAMP-hydrolytic activity. Overexpression of Rv0805 has been used as a tool to lower intracellular cAMP levels and thereby elucidate the roles of cAMP in mycobacteria. Here we show that levels of cAMP in M. tuberculosis were lowered by only .30% following overexpression of Rv0805, and transcript levels of a number of genes, which include those associated with virulence and the methyl citrate cycle, were altered. The genes that showed altered expression were distinct from those differentially regulated in a strain deleted for the cAMP-receptor protein (CRP(Mt)), consistent with the relatively low dependence on cAMP of CRP(Mt) binding to DNA. Using mutants of Rv0805 we show that the transcriptional signature of Rv0805 overexpression is a combination of catalysis-dependent and independent effects, and that the structurally flexible C-terminus of Rv0805 is crucial for the catalysis-independent effects of the protein. Our study demonstrates the dissociation of Rv0805 and cAMP-regulated gene expression, and reveals alternate functions for this phosphodiesterase from M. tuberculosis. [Data is also available from ]
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-BUGS-146
refinement:
raw
keywords:
functional genomics
dateModified:
06-26-2014
creators:
Adam Witney
availability:
available
types:
gene expression
name:
Mycobacterium tuberculosis
name:
strain or line design
ID:
A-BUGS-23
name:
BuG@S M.tuberculosis / M.bovis 10944 TBv2_1_1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-BUGS-146/E-BUGS-146.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-BUGS-146/E-BUGS-146.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress