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Title: Gene expression changes of Streptomyces coelicolor M145 under external and internal disturbances      
dateReleased:
12-31-2013
description:
The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling variation of S. coelicolor M145, ΔscbR2 mutation with jadomycin addition at 30 h.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-53563
refinement:
raw
alternateIdentifiers:
53563
keywords:
functional genomics
dateModified:
01-13-2014
availability:
available
types:
gene expression
name:
Streptomyces coelicolor
ID:
A-GEOD-18100
name:
NimbleGen Streptomyces coelicolor 12 × 135k array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53563/E-GEOD-53563.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53563/E-GEOD-53563.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53563
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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