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Title: Epigenome study of normal human epithelial tissues      
dateReleased:
01-14-2014
description:
Genome wide DNA methylation profiling of normal human epithelial tissues of breast colon lung and endometrial origin, WBC and sperm to identify breast-specific methylated gene promoter loci. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs. Breast tissues were pre-enriched for organoids and other epithelial tissues were carefully selected by the pathologist. Bisulphite converted DNA from the 23 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2. Two arrays of 12 and samples each were run separately. Each array included a control of human sperm DNA treated with SssI CpG methylase and human sperm DNA not treated (one sample did not pass QC and was dicarded ) .
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-54025
refinement:
raw
alternateIdentifiers:
54025
keywords:
functional genomics
dateModified:
06-03-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-8490
name:
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-54025/E-GEOD-54025.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-54025/E-GEOD-54025.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE54025
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress