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Title: Characterization of Gene Expression in liver cell      
dateReleased:
01-31-2014
description:
The Human Agilent 60-mer, oligonucleotide microarray consists of a total of probes covering 41,096 genes of human. Through a validation study, total RNA isolated from liver cells were used for comparison of gene expression profiles via microarray examination. A list of significantly differentially expressed genes was generated; 267 genes were up-regulated genes, and 65 genes were down-regulated genes. The Agilent microarray platform is based upon the hybridization of a single labeled sample (derived from RNA), followed by one-channel detection. The intensity of the hybridization signal is used to determine target concentration. In order to validate the technical quality of each probe in our arrays, we carried out 6 independent hybridizations on samples. To examine the gene expression profiles between rice root and shoot development, total RNA extracted from cell lines were processed on the Agilent microarray following the standard protocol using five arrays for each tissue type. Raw expression data from 6 microarrays were normalized and Pearson's correlation coefficients were calculated for the data sets of hybridization signal intensities. Oxaliplatin-resistant MHCC97H liver cell line treated with Oxaliplatin or control.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-51951
refinement:
raw
alternateIdentifiers:
51951
keywords:
functional genomics
dateModified:
07-23-2015
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-AGIL-28
name:
Agilent Whole Human Genome Microarray 4x44K 014850 G4112F (85 cols x 532 rows)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-51951/E-GEOD-51951.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-51951/E-GEOD-51951.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51951
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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