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Title: Identification of biomarkers for Miltefosine resistance in Leishmania donovani      
dateReleased:
01-31-2014
description:
To identify biomarkers specific for Miltefosin resistant parasites, we used DNA microarray to identify differentially expressed genes in resistant parasite in comparision to sensitive and further validated with real time PCR assay and immunoblotting assay Clinically proven miltefosine reistant as well as respondant parasite strains isolated from splenic aspirates and grown in M199 media. For microarray resistant and sensitive samples run in duplicate while for real time PCR, 25 resistant and 33 sensitive strains used. For immunoblotting, confirmed genes were cloned and recombinant protein blotted against miltefosine failure and treated patients sera.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-53738
refinement:
raw
alternateIdentifiers:
53738
keywords:
functional genomics
dateModified:
06-03-2014
availability:
available
types:
gene expression
name:
Leishmania donovani
ID:
A-GEOD-18125
name:
Leishmania donovani Gene Expression 8x15k Microarray - Genotypic Technology Private Limited (AMADID: 035638) (condensed)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53738/E-GEOD-53738.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53738/E-GEOD-53738.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53738
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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