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Title: Genome-scale reconstruction of the sigma factor network in E. coli      
dateReleased:
02-05-2014
description:
We report the application of single-molecule-based sequencing technology for high-throughput profiling of transcription start sites for Escherichia coli under different conditions. By obtaining sequence from 5' RACE (rapid amplification of cDNA ends) followed by deep sequencing, we generated genome-wide TSS (transcription start site) maps for E. coli. This TSS-map was integrated with ChIP-chip data generated for 6 sigma factors in E. coli, resulting in reconstruction of sigma factor network in E. coli. Examination of transcription start sites by biological duplicates from E. coli for 3 different conditions
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-46737
refinement:
raw
alternateIdentifiers:
46737
keywords:
functional genomics
dateModified:
05-06-2014
availability:
available
types:
gene expression
name:
Escherichia coli str. K-12 substr. MG1655
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46737/E-GEOD-46737.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46737/E-GEOD-46737.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46737
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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