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Title: Transcription profiling by array of Arabidopsis thaliana plants from the genotypes wild type and abi2-2T subjected to mild light stress      
dateReleased:
02-10-2014
description:
Arabidopsis thaliana plants from the genotypes Col-0 and abi2-2T (triple mutant abi1-2/abi2-2/hab1-1) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levingtons L2+S. On the day of the experiment, two hours after the lights turned on they were subjected to mild light stress (approx. 1500 umol*m-2*s-1, measured with a PAR light meter) for 30 min under an Isolight machine (white LED array), and immediately snap-frozen in liquid nitrogen. The outer leaves from four biological replicates were ground and RNA purified, and labelled RNA was produced with the Agilent Quick Amp kit. Labelled RNA was hybridized to Agilent Arabidopsis V4 microarray slides, and the fluorescence recorded with a Genepix scanner.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-2047
refinement:
raw
keywords:
functional genomics
dateModified:
06-04-2014
availability:
available
types:
gene expression
name:
Arabidopsis thaliana
ID:
A-MEXP-1523
name:
Agilent Arabidopsis V4 4x44K 021169 G2519F (GenePix layout)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2047/E-MTAB-2047.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2047/E-MTAB-2047.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress