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Title: Overexpression of GmDof4 from Soybean Significantly Enhances the Lipid Content of Chlorella ellipsoidea      
dateReleased:
04-16-2014
description:
Here, we transferred GmDof4 from soybean (Glycine max), a transcription factor affecting content of lipid in Arabidopsis, into C. ellipsoidea and found that the expression of GmDof4 significantly enhanced the lipid content by maximum 57.21%, shifted the content of different fatty acids, but did not affect the growth of the host cells. Using the Solexa/Illumina-based RNA-Seq analysis, we found expression of GmDof4 significantly regulates 1,076 genes, of which 754 genes were up-regulated and 322 genes were down-regulated, under mixotrophic culture. This study provides a new way to improve the lipid of microalgae. The GmDof4 transgenic strain Dof4-1 and control (pCK transgenic line CK-1) were cultured in liquid antibiotic-free Endo medium at 25℃ under illumination (100 μmol photons m-2 s-1) for 120 h. Cells at a concentration of approximately 1× 10^7 cells/mL were collected for library construction and sequencing using Illumina GAIIx.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-37473
refinement:
raw
alternateIdentifiers:
37473
keywords:
functional genomics
dateModified:
05-31-2014
availability:
available
types:
gene expression
name:
Pseudochlorella pringsheimii
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-37473/E-GEOD-37473.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-37473/E-GEOD-37473.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37473
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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