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Title: RNA sequencing of two protein phosphatases(ppm2 and ppm5) knockouts in Plasmodium berghei to study their role in the parasite's sexual development      
dateReleased:
04-16-2014
description:
To identify protein phosphatases (PP) that are essential during asexual blood stage development in Plasmodium berghei, we attempted systematic deletion of the 30 P. berghei PP genes using double homologous recombination. Of these, we performed strand-specific RNA-sequencing (RNA-Seq) analysis for 2 myristoylated PP mutants _ppm2 and _ppm5 lines across relevant lifestages ( activated gametocyte and schizont stages in both _ppm2 and _ppm5 and ookinete stage in _ppm5 alone). Two to four biological replicates each for _ppm2, _ppm5 and wild-type parasites were processed into strand-specific RNA-seq libraries and sequenced in Illumina Hiseq platform using 100-bp paired end chemistry.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-2215
refinement:
raw
keywords:
functional genomics
dateModified:
05-03-2014
creators:
Abhinay Ramaprasad
availability:
available
types:
gene expression
name:
Plasmodium berghei
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2215/E-MTAB-2215.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2215/E-MTAB-2215.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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