Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Identification of Novel Post-Transcriptional Features in Olfactory Receptor Family mRNAs      
dateReleased:
05-13-2014
description:
Olfactory receptor (Olfr) genes comprise the largest gene family in mice. Despite their importance, most Olfr mRNAs are uncharacterized. Using RNA-seq analysis, we annotated the repertoire of mouse Olfr mRNAs expressed in the olfactory epithelium and found that they have several atypical features suggesting how post-transcriptional regulation impacts their expression. First, many Olfr mRNAs are intronless and those with introns typically have a single intron at the 5’ end. Second, Olfr mRNAs, as a group, have dramatically higher average AU content and lower predicted secondary structure than do control mRNAs. Third, Olfr mRNAs have a higher density of AU-rich elements (AREs) than control mRNAs. Fourth, Olfr mRNAs have much shorter 3’ UTR regions and fewer predicted binding sites for neurally expressed miRNAs than control mRNAs. Fifth, Olfr mRNAs have a significantly greater number of upstream open reading frames (uORFs) in their 5’ UTR than control mRNAs. All of these novel properties correlated with higher Olfr expression. Finally, we identified striking differences in the mRNAs from the two classes of Olfr genes, a finding consistent with their independent evolutionary origin. Our study suggests that the Olfr gene family has encountered unusual selective forces that have driven these unique post-transcriptional regulatory features. Examine transcriptome of Olfr genes in mouse olfactory epithelium
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-57567
refinement:
raw
alternateIdentifiers:
57567
keywords:
functional genomics
dateModified:
08-13-2014
availability:
available
types:
gene expression
name:
Mus musculus
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57567/E-GEOD-57567.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57567/E-GEOD-57567.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57567
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
Similar Datasets

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.